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Biotechnology has helped farmers to get pest resistant cotton crops. Explain the technique adopted along with its mode of action

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by Rising star (791 points)

Its a great privilege for us that pestie resistant plant can be formed by using biotechnology tool. In this process we can add pest resistant gene to the plant vector and get insert to the concerned / host plant. 

There is novel approach using RNAi (RNA interference) we can make pest resistant plant.

Several nematodes parasitise a wide variety of plants and animals including human beings. A nematode Meloidegyne incognitia infects the roots of tobacco plants and causes a great reduction in yield. A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference (RNAi). 

Basically RNAi takes place in all eukaryotic organisms. we can say this is a method of cellular defense. This method involves silencing of a specific mRNA due to a complementary dsRNA molecule which binds to and prevents translation of the mRNA (silencing).

Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant. The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA’s being complementary to each other formed a double stranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant therefore got itself protected from the parasite.

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